Unlocking the Potential: A Comprehensive Guide to Scalable Recombinant Protein Production in Escherichia Coli

Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli (Woodhead Publishing Series in Biomedicine Book 42)

by Jay da Silva

4 out of 5

Language	:	English
File size	:	2765 KB
Text-to-Speech	:	Enabled
Screen Reader	:	Supported
Enhanced typesetting	:	Enabled
Print length	:	188 pages
Hardcover	:	510 pages
Item Weight	:	1.95 pounds
Dimensions	:	6.14 x 1.13 x 9.21 inches

FREE

Recombinant protein production has revolutionized the biotechnology industry, enabling the mass production of therapeutic proteins, enzymes, and other valuable biomolecules. Escherichia coli (E. coli),a versatile and well-characterized bacterium, has emerged as a preferred host for recombinant protein production due to its rapid growth rate, genetic tractability, and ability to express high levels of foreign proteins. However, scaling up recombinant protein production in E. coli poses unique challenges that require careful consideration and expertise.

To address these challenges, "Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli" provides a comprehensive and practical roadmap for researchers and industry professionals alike. This comprehensive guide covers all aspects of recombinant protein production in E. coli, from strain selection and expression system design to scale-up strategies and purification techniques.

Key Features

• In-depth coverage of strain selection, expression systems, and fermentation strategies.
• Detailed protocols for protein expression, purification, and characterization.
• Troubleshooting guide for common problems encountered in recombinant protein production.
• Case studies and expert insights from industry leaders.

Target Audience

This practical guide is an invaluable resource for:

• Researchers in academia and industry involved in recombinant protein production.
• Bioprocess engineers and scientists responsible for scale-up and production.
• Students pursuing degrees in biotechnology, biochemistry, and related fields.

Chapter Overview

Chapter 1: to Recombinant Protein Production in E. coli

• Overview of recombinant protein production.
• Advantages and challenges of using E. coli as a host.
• Applications of recombinant proteins in various industries.

Chapter 2: Strain Selection and Expression Systems

• Criteria for strain selection.
• Types of expression systems and their advantages/disadvantages.
• Optimization of expression conditions.

Chapter 3: Fermentation Strategies for Scalable Production

• Bioreactor design and configuration.
• Media optimization for high cell density and protein yields.
• Monitoring and control of fermentation parameters.

Chapter 4: Protein Expression and Purification

• Induction strategies for protein expression.
• Downstream processing techniques for protein purification.
• Characterization of recombinant proteins.

Chapter 5: Troubleshooting and Case Studies

• Common problems encountered in recombinant protein production.
• Troubleshooting strategies and solutions.
• Case studies of successful recombinant protein production scale-up.

"Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli" empowers researchers and industry professionals with the knowledge and expertise necessary to successfully navigate the complexities of recombinant protein production. By providing a comprehensive and practical roadmap, this guide accelerates the development and commercialization of valuable biomolecules, ultimately contributing to advancements in healthcare, biotechnology, and beyond.

To Free Download your copy of "Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli," visit our website or contact your local bookstore. Unleash the full potential of recombinant protein production and revolutionize your research and development endeavors.

Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli (Woodhead Publishing Series in Biomedicine Book 42)

by Jay da Silva

4 out of 5

Language	:	English
File size	:	2765 KB
Text-to-Speech	:	Enabled
Screen Reader	:	Supported
Enhanced typesetting	:	Enabled
Print length	:	188 pages
Hardcover	:	510 pages
Item Weight	:	1.95 pounds
Dimensions	:	6.14 x 1.13 x 9.21 inches

FREE